A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain.
Methods Enzymol
; 437: 135-59, 2008.
Article
em En
| MEDLINE
| ID: mdl-18433627
ABSTRACT
Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Transporte de Elétrons
/
Óxido Nítrico
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2008
Tipo de documento:
Article