Design, synthesis, and evaluation of an isotopic labeling strategy for studying fatty acid-protein binding by NMR.
Mol Biosyst
; 4(6): 551-7, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18493652
ABSTRACT
Here we describe the synthesis and evaluation of a new isotopic labeling strategy for fatty acids to be used as probes for studying ligand binding by NMR. We synthesized palmitic acid with carbons C-3 through C-16 perdeuterated, C-1 and C-2 with 13C atoms and hydrogens at C-2. Our strategy began with commercially available perdeuterated myristic acid and built up to palmitic acid using a Horner-Wadsworth-Emmons reaction. To evaluate the power of this isotopic enrichment strategy, we evaluated ligand binding to a prototypical member of the intracellular lipid binding protein family, FABP2. This small 15 kDa protein is well known to bind fatty acids with high affinity, and we used this system to illustrate the spectral filtering abilities of our described labeling strategy. Herein we show how having two vicinal 13C-enriched carbons, two hydrogens at the alpha-position, and a perdeuterated aliphatic tail allows the efficient use of multidimensional NMR experiments to effectively filter all background resonances from the protein and facilitate the study of ligand binding.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectroscopia de Ressonância Magnética
/
Ácido Palmítico
/
Proteínas de Ligação a Ácido Graxo
/
Marcação por Isótopo
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Mol Biosyst
Ano de publicação:
2008
Tipo de documento:
Article