Involvement of the vascular wall in regenerative processes after CA1 ischemic neuronal death.
Int J Dev Neurosci
; 26(6): 541-50, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18573330
ABSTRACT
The involvement of vascular wall in response to neuronal death was challenged here using a transient forebrain ischemia model in gerbil, which causes CA1 neuronal death and trigger neurogenesis in hippocampus. We found an important vascular reaction in CA1 5 days after ischemia evaluated by Von Willebrand factor and Vimentin immunoreactivity, as well as increased expression of angiogenic and neurogenic regulators Vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2). Analysing the morphology and cell phenotype by confocal microscopy, we confirmed the colocalization of the neurogenic markers (bromodeoxyuridine-neuronal nuclei-TOPRO-3) in newborn cells associated to vascular walls in CA1 and dentate gyrus of hippocampus 32 days after ischemia. The results indicate that vascular tissues may participate in neurogenesis after brain ischemia, reinforce the notion that blood vessels represent a source of neuronal progenitor cells in damaged brain areas and suggest that molecular and cellular manipulation of the vascular wall may expand the possibilities of novel regenerative therapies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Artérias Cerebrais
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Ataque Isquêmico Transitório
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Proliferação de Células
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Células-Tronco Adultas
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Hipocampo
/
Neurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Int J Dev Neurosci
Ano de publicação:
2008
Tipo de documento:
Article