Bone marrow stromal cells spontaneously produce Flt3-ligand: influence of ionizing radiations and cytokine stimulation.
Int J Radiat Biol
; 84(8): 659-67, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18608642
ABSTRACT
PURPOSE:
To define the ability of human bone marrow (BM) stromal cells to produce fms-like tyrosine kinase 3 (Flt3)-ligand (FL), and the effect of irradiation, tumour necrosis factor-alpha (TNFalpha) or tumour growth factor beta (TGFbeta) on FL production. MATERIAL ANDMETHODS:
Primary BM stromal cell cultures were irradiated at 2-10 Gy or were stimulated with TNFalpha or TGFbeta1. The presence of FL was tested in culture supernatants and in cell lysate. The presence of a membrane-bound form of FL and the level of gene expression were also tested.RESULTS:
Primary BM stromal cells spontaneously released FL. This production was increased by TNFalpha but not by TGFbeta1 or by irradiation. Chemical induction of osteoblastic differentiation from BM stromal cells also induced an increase in FL release.CONCLUSIONS:
Our results suggest that the observed increase in FL concentration after in vivo irradiation is an indirect effect. The possible implication of BM stromal cells in these mechanisms is discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
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Fator de Necrose Tumoral alfa
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Fator de Crescimento Transformador beta1
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Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
Int J Radiat Biol
Ano de publicação:
2008
Tipo de documento:
Article