Spectroscopic studies of the chlorophyll d containing photosystem I from the cyanobacterium, Acaryochloris marina.
Biochim Biophys Acta
; 1777(11): 1400-8, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-18817746
ABSTRACT
Absorbance difference spectroscopy and redox titrations have been applied to investigate the properties of photosystem I from the chlorophyll d containing cyanobacterium Acaryochloris marina. At room temperature, the (P740(+)-P740) and (F(A/B)(-)-F(A/B)) absorbance difference spectra were recorded in the range between 300 and 1000 nm while at cryogenic temperatures, (P740(+)A(1)(-)-P740A(1)) and ((3)P740-P740) absorbance difference spectra have been measured. Spectroscopic and kinetic evidence is presented that the cofactors involved in the electron transfer from the reduced secondary electron acceptor, phylloquinone (A(1)(-)), to the terminal electron acceptor and their structural arrangement are virtually identical to those of chlorophyll a containing photosystem I. The oxidation potential of the primary electron donor P740 of photosystem I has been reinvestigated. We find a midpoint potential of 450+/-10 mV in photosystem I-enriched membrane fractions as well as in thylakoids which is very similar to that found for P700 in chlorophyll a dominated organisms. In addition, the extinction difference coefficient for the oxidation of the primary donor has been determined and a value of 45,000+/-4000 M(-1) cm(-1) at 740 nm was obtained. Based on this value the ratio of P740 to chlorophyll is calculated to be 1 to approximately 200 chlorophyll d in thylakoid membranes. The consequences of our findings for the energetics in photosystem I of A. marina are discussed as well as the pigment stoichiometry and spectral characteristics of P740.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral
/
Clorofila
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Cianobactérias
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Complexo de Proteína do Fotossistema I
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2008
Tipo de documento:
Article