Effects of human PrPSc type and PRNP genotype in an in-vitro conversion assay.
Neuroreport
; 19(18): 1783-6, 2008 Dec 03.
Article
em En
| MEDLINE
| ID: mdl-18955905
ABSTRACT
Prion protein type and codon 129 genotype are thought to be major determinants of susceptibility and phenotype in human prion diseases. Using an in-vitro system (protein misfolding cyclic amplification) we have attempted to model human prion protein conversion using the abnormal prion protein associated with each of the major sporadic Creutzfeldt-Jakob disease subtypes, in substrates containing the normal cellular form of the prion protein of each of the three possible human PRNP codon 129 polymorphic genotypes. The prion protein type is converted with fidelity in these amplification reactions, but the efficiency of conversion depends both on the methionine/valine polymorphic status of the sporadic Creutzfeldt-Jakob disease seed and substrate homogenate, and on the abnormal prion protein type.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Príons
/
Síndrome de Creutzfeldt-Jakob
/
Proteínas PrPSc
/
Lobo Frontal
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Neuroreport
Ano de publicação:
2008
Tipo de documento:
Article