A method for making histological preparations equivalent to semithin sections with large examination areas for multipurpose morphological studies.

ABSTRACT

Effective methods for making semithin sections of large surface area are not currently available. An alternative solution to this problem, without actual histological section preparation, is proposed. This is a modification of a combination of methods for fixing tissues and embedding them in epoxide resin using known technical methods. This method for making preparations for microscopic examination of biological objects with large surface areas has additional valuable potential, as it allows investigations of a variety of complexes of soft and hard (cartilaginous and bony) tissues without prior decalcination.