Evaluation of two gene-silencing constructs for resistance to tomato yellow leaf curl viruses in Nicotiana benthamiana plants.
Acta Virol
; 52(3): 143-9, 2008.
Article
em En
| MEDLINE
| ID: mdl-18999888
ABSTRACT
Infiltration of Agrobacterium tumefaciens into intact plant leaves of N. benthamiana was used to test the efficiency of two virus-based silencing constructs conferring resistance to the closely related begomoviruses. The constructs contained the most conserved sequences of the coat protein (CP) gene and replication-associated protein (Rep) gene of Tomato yellow leaf curl Sardinia virus (Sicily strain) (TYLCSV-[Sic]). Both constructs formed a hairpin structure that enhanced the post-transcriptional gene-silencing mechanism. When agro-infiltrated plants were challenged separately with infectious viruses TYLCSV-[Sic] and Tomato yellow leaf curl virus (TYLCV), the plants showed resistance to TYLCSV-[Sic], but not to the related TYLCV.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Nicotiana
/
Proteínas Virais
/
Solanum lycopersicum
/
Interferência de RNA
/
Agricultura
/
Begomovirus
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Acta Virol
Ano de publicação:
2008
Tipo de documento:
Article