Effect of malathion on Bufo arenarum Hensel development--I. Esterase inhibition and recovery.
Biochem Pharmacol
; 41(4): 491-5, 1991 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-1900004
ABSTRACT
Newly fertilized Bufo arenarum Hensel embryos were exposed continuously or for a brief period (72-120 hr) to malathion (44 ppm) and then resuspended in amphibian Ringer's solution. Continuous exposure depressed acetylcholinesterase (EC 3.1.1.7), butyrylcholinesterase (EC 3.1.1.8) and carboxylesterase (EC 3.1.1.1) activities. The activities of the three enzymes in embryos treated for 72 hr recovered after a delay of 24 hr, but these enzymes showed different rates of recovery in embryos treated for 120 hr. Acrylamide disc electrophoresis showed several bands of esterase activity in control embryos. Continuous exposure to malathion abolished all esterase activity within 48 hr, but if the exposure continued new bands of esterase activity appeared at 120 hr of exposure. The zymograms of embryos exposed for 72 or 120 hr to malathion and then transferred to uncontaminated medium for 120 hr were similar to that of control embryos.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esterases
/
Malation
Limite:
Animals
Idioma:
En
Revista:
Biochem Pharmacol
Ano de publicação:
1991
Tipo de documento:
Article