Prevalence of pfmdr1, pfcrt, pfdhfr and pfdhps mutations associated with drug resistance, in Luanda, Angola.
Malar J
; 7: 236, 2008 Nov 17.
Article
em En
| MEDLINE
| ID: mdl-19014684
ABSTRACT
BACKGROUND:
Malaria is the infectious disease causing the highest morbidity and mortality in Angola and due to widespread chloroquine (CQ) resistance, the country has recently changed its first-line treatment recommendations for uncomplicated malaria, from CQ to artemisinin combination therapies (ACT) in adults, and sulphadoxine/pyrimethamine (S/P) in pregnant women. Loss of SP sensitivity is, however, progressing rapidly in Africa and, in this study, were investigated a number of molecular markers associated to CQ and S/P.METHODS:
Blood samples were collected from 245 children with uncomplicated malaria, admitted at the Pediatric Hospital Dr. David Bernardino (HPDB), Angola, and the occurrence of mutations in Plasmodium falciparum was investigated in the pfmdr1 (N86Y) and pfcrt (K76T) genes, associated with CQ resistance, as well as in pfdhfr (C59R) and pfdhps (K540E), conferring SP resistance.RESULTS:
The frequencies of pfmdr1 mutations in codon 86 were 28.6% N, 61.3% Y and 10.1% mixed infections (NY). The frequency of pfcrt mutations in codon 76 were 93.9% K, 5.7% T and 0.4% mixed infections (KT). For pfdhfr the results were in codon 59, 60.6% C, 20.6% R and 18.8% mixed infections (CR). Concerning pfdhps, 6.3% of the isolates were bearers of the mutation 540E and 5.4% mixed infections (K540E).CONCLUSION:
The results of this epidemiologic study showed high presence of CQ resistance markers while for SP a much lower prevalence was detected for the markers under study.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
/
4_TD
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Plasmodium falciparum
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Tetra-Hidrofolato Desidrogenase
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Resistência a Medicamentos
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Proteínas de Protozoários
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Malária Falciparum
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Proteínas Associadas à Resistência a Múltiplos Medicamentos
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Di-Hidropteroato Sintase
Tipo de estudo:
Guideline
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Adolescent
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Animals
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Child
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Child, preschool
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Humans
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Infant
País/Região como assunto:
Africa
Idioma:
En
Revista:
Malar J
Ano de publicação:
2008
Tipo de documento:
Article