Purification of astaxanthin from mutant of Phaffia rhodozyma JH-82 which isolated from forests trees of Iran.
Pak J Biol Sci
; 10(5): 802-5, 2007 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-19069868
ABSTRACT
Astaxanthin have been extracted and purified from mutant isolate of Phaffia rhodozyma JH-82. Purified astaxanthin was identified by spectrophotometric, TLC and HPLC analysis and were compared with synthetic astaxanthin. Results of TLC analysis indicated that isolate of P. rhodozyma JH-82 were able to produce nine different carotenoids and high level of carotenoids was belong to astaxanthin. Results of this study for pure astaxanthin production indicated that mutant of JH-82 of P. rhodozyma (230 microg g(-1)(dried yeast)) produced more astaxanthin than natural isolate JH-80 (140 microg g(-1)(dried yeast)). The HPLC spectrum showed retention time 11 min for both purified and synthetic astaxanthin and solvent was CDCl3.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Árvores
/
Basidiomycota
/
Mutação
País/Região como assunto:
Asia
Idioma:
En
Revista:
Pak J Biol Sci
Ano de publicação:
2007
Tipo de documento:
Article