[Identification and quantitative analysis of Archaea involved in periodontal disease].
Zhonghua Kou Qiang Yi Xue Za Zhi
; 43(10): 589-91, 2008 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-19087619
ABSTRACT
OBJECTIVE:
To make qualitative and quantitative analysis of Archaea in subgingival plaque sample and to investigate the relationship between periodontal disease and Archaea.METHODS:
Subgingival plaque was collected from 23 patients with aggressive periodontitis, 29 with chronic periodontitis, 35 with plaque-induced gingivitis and 38 healthy controls. Qualitative and quantitative analysis of methanogenic archaea was performed by amplification of the 16S rRNA genes in the DNA extracted from the plaque samples.RESULTS:
Archaea were found in 65% of aggressive periodontitis patients, 72% of chronic periodontitis, 26% of gingivitis and zero of healthy subjects. Quantitative analysis showed the average abundance of archaeal 16S rRNA gene in Archaea-positive patients was different among the three groups. The average 16S rRNA gene copy number from per microg wet plaque was 6.66 x 10(6) in aggressive periodontitis sufferers, 4.47 x 10(6) in chronic periodontitis and 1.78 x 10(6) in gingivitis groups. The prevalence of Archaea and the average Archaea 16S rRNA gene numbers in periodontitis groups were higher than those in gingivitis group (P < 0.05).CONCLUSIONS:
This suggests that Archaea may be implicated as causative agents for periodontitis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Periodontais
/
Archaea
/
Placa Dentária
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Qualitative_research
/
Risk_factors_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Kou Qiang Yi Xue Za Zhi
Ano de publicação:
2008
Tipo de documento:
Article