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A new procalcitonin optical immunosensor for POCT applications.
Baldini, F; Bolzoni, L; Giannetti, A; Kess, M; Krämer, P M; Kremmer, E; Porro, G; Senesi, F; Trono, C.
Afiliação
  • Baldini F; Institute of Applied Physics, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy. f.baldini@ifac.cnr.it
Anal Bioanal Chem ; 393(4): 1183-90, 2009 Feb.
Article em En | MEDLINE | ID: mdl-19096832
A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the non-specific adsorption. A limit of detection of 0.088 mg L(-1) was achieved within the working range of 0.28-50 mg L(-1) in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L(-1) were the attained limit of detection and working range, respectively.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Precursores de Proteínas / Calcitonina / Técnicas Biossensoriais / Sistemas Automatizados de Assistência Junto ao Leito Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Precursores de Proteínas / Calcitonina / Técnicas Biossensoriais / Sistemas Automatizados de Assistência Junto ao Leito Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2009 Tipo de documento: Article