A new procalcitonin optical immunosensor for POCT applications.
Anal Bioanal Chem
; 393(4): 1183-90, 2009 Feb.
Article
em En
| MEDLINE
| ID: mdl-19096832
A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the non-specific adsorption. A limit of detection of 0.088 mg L(-1) was achieved within the working range of 0.28-50 mg L(-1) in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L(-1) were the attained limit of detection and working range, respectively.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Calcitonina
/
Técnicas Biossensoriais
/
Sistemas Automatizados de Assistência Junto ao Leito
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2009
Tipo de documento:
Article