Construction and tropism characterisation of recombinant viruses exhibiting HIV-1 env gene from seminal strains.
Virology
; 386(2): 373-9, 2009 Apr 10.
Article
em En
| MEDLINE
| ID: mdl-19232661
ABSTRACT
Genetic differences between blood and mucosal-derived HIV-1 strains have been widely reported. As amplification of HIV-1 strains from mucosal samples including semen or saliva by co-culture has low sensitivity, we developed the construction of chimeric viruses expressing wild-type seminal HIV-1 envelope protein. Chimeric viruses were produced by co-transfection of a V1-V3 deleted pNL 43 vector and PCR fragments spanning the deleted region, amplified from HIV-1 RNA positive seminal plasma samples. After an initial testing of co-receptor usage by a tropism recombinant test, replication capacity and amplification of these recombinant viruses were assessed using PBMC. Four chimeric replicative strains, all using CXCR4 as coreceptor, were produced. The interaction between cell-free viral particles and reporter cell lines was assessed by confocal microscopy. These replicative chimeras exhibiting HIV-1 env from seminal strains represent useful tools for the in vitro study of the heterosexual transmission of HIV-1 and testing of microbicide activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes env
/
HIV-1
/
Produtos do Gene env do Vírus da Imunodeficiência Humana
Limite:
Humans
/
Male
Idioma:
En
Revista:
Virology
Ano de publicação:
2009
Tipo de documento:
Article