Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis.
Crit Care Med
; 37(3): 1038-45, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19237915
ABSTRACT
OBJECTIVE:
To examine peripheral leukocyte Dectin-1 regulation in clinically relevant models of fungal and polymicrobial sepsis.DESIGN:
Prospective animal study.SETTING:
University medical school research laboratory.SUBJECTS:
Age, weight, and sex matched ICR/HSD mice.INTERVENTIONS:
Mice were infected with Candida albicans (1 x 10, intravenously) or were subjected to cecal ligation and puncture to induce polymicrobial sepsis. MEASUREMENTS Blood, spleen, and peritoneal exudate were harvested and leukocytes were isolated. Leukocytes were evaluated for membrane-associated Dectin-1 expression and cell phenotype by flow cytometry. MAINRESULTS:
In C. albicans infection, Dectin-1-positive blood and splenic leukocytes were increased from 23.5% to 58.9% over the course of infection. The increased percentage of Dectin-1-expressing cells was primarily attributable to neutrophilia. However, the amount of Dectin-1 expressed by blood and splenic neutrophils in C. albicans-infected mice was decreased by a range of 49.0% to 53.3%. C. albicans infection also resulted in an infiltration of Dectin-1-positive macrophages and neutrophils into the kidney. In contrast, polymicrobial sepsis decreased blood leukocyte Dectin-1-expressing cells by up to 51.4%. This reduction was due to a decrease in Dectin-1-positive neutrophils in the periphery. However, the percentage of Dectin-1-expressing cells in the peritoneal cavity increased by 774% with cecal ligation and puncture. Treatment of isolated neutrophils with three soluble glucans, mannan, lipopolysaccharide, or a variety of cytokines revealed that glucans, alone or in combination, were the only treatment that resulted in a decrease in Dectin-1-positive neutrophils.CONCLUSIONS:
We conclude that peripheral leukocyte Dectin-1 expression is differentially regulated in fungal vs. polymicrobial sepsis. These data demonstrate that leukocyte Dectin-1 levels are modulated in response to infections of fungal and nonfungal origin.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fungemia
/
Bacteriemia
/
Leucócitos
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Proteínas de Membrana
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Crit Care Med
Ano de publicação:
2009
Tipo de documento:
Article