[An evaluation of the conformational changes in the superhelical DNA of eukaryotic cells by direct nucleoid fluorometry. II. The characteristics of the change in acridine orange fluorescence in studying the superhelical DNA of rat thymocytes]. / Otsenka konformatsionnykh izmenenii superspiral'noi DNK éukarioticheskikh kletok metodom priamoi fluorimetrii nukleoidov. II. Osobennosti izmeneniia fluorestsentsii akridinovogo oranzhevogo pri izuchenii superspiral'noi DNK timotsitov krys.

Comparative studies of acridine orange (AO) and ethidium bromide interactions with supercoiled DNA (scDNA) of thymocytes were performed in which various conformational changes were induced. AO may be efficiently used for evaluation of conformational alterations of scDNA. Moreover, employing the maximum values of AO fluorescence allows to determine the maximum levels of scDNA relaxation.