[Individual identification and paternity testing of bulls using microsatellite].
Yi Chuan
; 31(3): 285-9, 2009 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-19273442
ABSTRACT
The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Impressões Digitais de DNA
/
Repetições de Microssatélites
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Yi Chuan
Ano de publicação:
2009
Tipo de documento:
Article