[Establishment of loop-mediated isothermal amplification for detection of Vibrio alginolyticus].
Fen Zi Xi Bao Sheng Wu Xue Bao
; 42(1): 70-6, 2009 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-19306691
ABSTRACT
Vibrio alginolyticus is the main Vibrio pathogen in aquaculture in the south of China. A one step loop-mediated isothermal amplification (LAMP) assay was developed for detection of Vibrio alginolyticus. A set of primers were designed from the OmpK sequence of Vibrio alginolyticus. The assay was optimised to amplify Vibrio alginolyticus DNA by incubation at 65 degrees C for only 1 h, and required only a simple water bath or heating block to provide a constant temperature of 65 degrees C. LAMP amplification products had a ladder-like appearance when electrophoresed on an agarose gel. The detection limit of the LAMP assay was n(cell) = 38/mL which was found to be higher than the commonly used PCR method. The assay was evaluated using clinical samples and the results indicated the suitability and simplicity of the test as a rapid, field diagnostic tool for Vibrio alginolyticus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Bacteriológicas
/
Técnicas de Amplificação de Ácido Nucleico
/
Vibrio alginolyticus
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Fen Zi Xi Bao Sheng Wu Xue Bao
Ano de publicação:
2009
Tipo de documento:
Article