Salivary gland thrombostasin isoforms differentially regulate blood uptake of horn flies fed on New Zealand White rabbits.
J Med Entomol
; 46(2): 351-7, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19351087
ABSTRACT
Thrombostasin (TS) is a previously characterized anticlotting protein with multiple isoforms found in the saliva of horn flies. In this report, the effect of TS isoforms on blood feeding was assessed using individual flies that carried corresponding ts allelles. Laboratory studies of horn fly blood feeding were conducted using colony-reared flies fed on New Zealand White (NZW) rabbits. After timed 20-min feeding periods, each fly was characterized for gender, blood volume uptake, and ts genotype. The results showed that mean blood volumes obtained by individual flies were not related to fly gender but were correlated to the ts genotype(s) carried by each fly. A fly having one or both ts alleles coding for the TS9 isoform took less blood than those possessing one or both ts alleles coding for the TS10 isoform. These results confirm the significant role that TS plays in horn fly feeding and highlight the differential impact of TS protein isoforms that vary by as few as three amino acids.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Muscidae
/
Caracteres Sexuais
/
Proteínas de Insetos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Med Entomol
Ano de publicação:
2009
Tipo de documento:
Article