Rapid method for detecting resistance to a QoI fungicide in Plasmopara viticola populations.
Pest Manag Sci
; 65(8): 840-3, 2009 Aug.
Article
em En
| MEDLINE
| ID: mdl-19418479
ABSTRACT
BACKGROUND:
The increasing occurrence of Qo inhibitor (QoI)-fungicide-resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide.RESULTS:
The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR-RFLP method. With this method, a glycine-to-alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan.CONCLUSION:
It is proposed that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for detecting azoxystrobin-resistant P. viticola populations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oomicetos
/
Pirimidinas
/
Farmacorresistência Fúngica
/
Fungicidas Industriais
/
Metacrilatos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Pest Manag Sci
Ano de publicação:
2009
Tipo de documento:
Article