Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.
Dev Dyn
; 238(6): 1398-46, 2009 Jun.
Article
em En
| MEDLINE
| ID: mdl-19441086
ABSTRACT
Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 2381398-1406, 2009. (c) 2009 Wiley-Liss, Inc.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xenopus
/
Mapeamento Cromossômico
/
Cromossomos
/
Mutação
Limite:
Animals
Idioma:
En
Revista:
Dev Dyn
Ano de publicação:
2009
Tipo de documento:
Article