Improved method for Duddingtonia flagrans chlamydospores production for livestock use.
Vet Parasitol
; 164(2-4): 344-6, 2009 Oct 14.
Article
em En
| MEDLINE
| ID: mdl-19497673
ABSTRACT
Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminthes in pasture. D. flagrans chlamydospores were produced using a two-step liquid/solid culture system. The inoculum grown in liquid medium was transferred to a rice substrate and kept at room temperature for 30 days. Grains were washed, filtered and centrifuged. The pellet was lyophilized and the obtained yield averaged 1 x 10(5) chlamydospores per gram of dried material. The lyophilized chlamydospores showed a trapping rate of 69% of infective larvae in vitro and were excreted entirely in ovine faeces. The results showed that most of the chlamydospores remained intact and viable after the lyophilization process.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Esporos Fúngicos
/
Controle Biológico de Vetores
/
Liofilização
/
Nematoides
Limite:
Animals
Idioma:
En
Revista:
Vet Parasitol
Ano de publicação:
2009
Tipo de documento:
Article