Calcineurin/NFAT pathway: a novel regulator of parturition.
Am J Reprod Immunol
; 62(1): 44-50, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19527231
ABSTRACT
PROBLEM:
The oxytocin (OT)-oxytocin receptor (OTR) system plays an important role in mammalian parturition. However, we found OTR-deficient (OTRKO) mice are fertile and deliver at term without birth defects, thus alternative pathways inducing parturition can be hypothesized. METHODS OF STUDY We tested the gene expression profile of OTRKO mice using suppressive subtractive hybridization, and focused on the calcineurin/nuclear factor of activated T cells (NFAT) pathway. We examined the expression and localization of this pathway in mouse parturition.RESULTS:
Calcineurin and NFATc1 were detected in the decidua of pregnant uteri at term using immunohistochemistry (IHC). We identified higher activation levels of NFATc1 in wild type (WT) than in OTRKO mice and increased calcineurin A and NFATc1 mRNA levels during pregnancy. Moreover, injection of FK506, the inhibitor of this pathway, prolonged the delivery of the first pup.CONCLUSION:
Our findings suggested that the calcineurin/NFAT pathway might play a substantial role in initiation of labor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calcineurina
/
Decídua
/
Parto
/
Fatores de Transcrição NFATC
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Am J Reprod Immunol
Ano de publicação:
2009
Tipo de documento:
Article