Evaluation of a high-intensity focused ultrasound-immobilized trypsin digestion and 18O-labeling method for quantitative proteomics.
Anal Chem
; 81(15): 6272-7, 2009 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-19555078
ABSTRACT
A new method that uses immobilized trypsin concomitant with ultrasonic irradiation results in ultrarapid digestion and more thorough (18)O labeling for quantitative protein comparisons. The method was reproducible and provided effective digestions within <1 min with lower amounts of enzyme, compared to traditional methods. This method was demonstrated for digestion of both simple and complex protein mixtures, including bovine serum albumin, a global proteome extract from the bacteria Shewanella oneidensis, and mouse plasma, as well as (18)O labeling of complex protein mixtures, validating this method for differential proteomic measurements. This approach is simple, reproducible, cost-effective, rapid, and well suited for automation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Isótopos de Oxigênio
/
Proteínas de Bactérias
/
Ultrassom
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Soroalbumina Bovina
/
Tripsina
/
Proteoma
/
Proteômica
Limite:
Animals
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2009
Tipo de documento:
Article