Fast and simple method for the determination of urinary 1-hydroxypyrene.
J Sep Sci
; 32(20): 3403-10, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19750508
ABSTRACT
A fast method for the measurement of metabolites of pyrene in urine was improved by HPLC with fluorescence detector using "heart-cut" technique. This method can quantify the total amount of pyrene metabolites corresponding to glucuronic acid and sulfate conjugates as well as free 1-OH-Py. The hydrolyzed biological fluid was directly injected into the chromatographic system, via a column-switching system. Pre-treatment and analysis were performed within 0.5 and 9 min, respectively. Enzymatic hydrolysis has been optimized to not exceed 2 h. The best response function, in the 0.2-10 ng/mL range, is the linear regression, bringing simplicity, good accuracy, and low LOQ. The intra- and interday precision values were inferior to 1 and 2%, respectively. The proposed method provided a simple, convenient, and practical procedure to determine the level of the main urinary pyrene metabolites in biological samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pirenos
/
Urina
/
Cromatografia Líquida de Alta Pressão
/
Mutagênicos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2009
Tipo de documento:
Article