Partial protection against SIV challenge by vaccination of adenovirus and MVA vectors in rhesus monkeys.
Gene Ther
; 17(1): 4-13, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-19759567
ABSTRACT
This study explores the effect of priming rhesus monkeys with an Ad5/35 vector expressing simian immunodeficiency virus (SIV) gag and gp120, and then boosting the animals with an modified vaccinia virus Ankara (MVA) vector encoding the same antigens after a 2-month interval. The animals were intravenously challenged with 100 TCID50 of highly pathogenic SIVmac239 virus 2 months after the booster vaccination. The priming vaccination induced robust SIV-specific cell-mediated and humoral immune responses, and boosting further enhanced the cellular immunity. Vaccination reduced peak and long-term viral loads by 1-2 logs for a period of >6 months, as reflected by a reduction in both the SIV RNA and DNA levels. Of considerable interest, the immunized monkeys did not suffer from loss of CD4 T cells, particularly central memory CD4 T cells. These results demonstrate that prophylactic vaccination with Ad5/35 followed by MVA reduces viral replication and prevents CD4 T-cell loss, and that these effects may decrease the likelihood of disease progression.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
4_TD
Base de dados:
MEDLINE
Assunto principal:
Vaccinia virus
/
Adenoviridae
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Síndrome de Imunodeficiência Adquirida dos Símios
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Imunização Secundária
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Vacinas contra a SAIDS
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Vetores Genéticos
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Gene Ther
Ano de publicação:
2010
Tipo de documento:
Article