2-Oxo-2H-benzo[h]benzopyran as a new light sensitive protecting group for neurotransmitter amino acids.
Amino Acids
; 39(1): 121-33, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-19908122
ABSTRACT
Aiming at the development of new benzopyran-based photocleavable protecting groups, novel chloromethylated and hydroxymethylated 2-oxo-2H-benzo[h]benzopyran derivatives bearing a methoxy substituent were designed and used in the synthesis of a series of fluorescent bioconjugates, by linking through an ester or urethane bond to several model neurotransmitter amino acids (glycine, alanine, beta-alanine and gamma-aminobutyric acid, GABA). The resulting fluorescent bioconjugates with emission in the visible range and high fluorescent quantum yields, were subjected to photocleavage reaction in methanol/HEPES buffer (8020) solution at different wavelengths of irradiation (250, 300, 350 and 419 nm) and photocleavage kinetic data were obtained.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neurotransmissores
/
Cumarínicos
/
Corantes Fluorescentes
/
Aminoácidos
/
Luz
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Amino Acids
Ano de publicação:
2010
Tipo de documento:
Article