Preparation, characterization and evaluation of a biopolymeric gold nanocomposite with antimicrobial activity.
Biotechnol Appl Biochem
; 55(1): 29-35, 2010 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-19929854
ABSTRACT
The present study describes the antimicrobial activity of C-AuNp-Amp (chitosan-capped gold nanoparticles coupled with ampicillin). C-AuNp-Amp was synthesized using the wet precipitation method and characterized using FTIR (Fourier-transform IR) spectroscopy and AFM (atomic force microscopy) techniques. The optimal level of ampicillin concentration that couples with the C-AuNp nanocomposite was determined by using UV-visible spectroscopy. The agar-well diffusion method was used to evaluate the antimicrobial activity, and the broth dilution assay was used to determine the MIC (minimum inhibitory concentration). The size of the ellipsoidal C-AuNp-Amp particles was found to be in the range of 50-100 nm. The FTIR spectrum confirms the bonding between amino groups of chitosan and carboxylic groups of ampicillin. The maximum coupling of ampicillin with C-AuNp was found to be 4.07 mg/10 ml. These results revealed the antimicrobial efficacy of C-AuNp-Amp and a 2-fold increase in activity was achieved when compared with that of free ampicillin. By reducing the antibiotic dosage to 50%, the side effects produced by antibiotics can be minimized.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Quitosana
/
Nanocompostos
/
Ouro
/
Ampicilina
/
Antibacterianos
Idioma:
En
Revista:
Biotechnol Appl Biochem
Ano de publicação:
2010
Tipo de documento:
Article