Rapid diagnostic method for the detection of diarrheagenic Escherichia coli by multiplex PCR.
Jpn J Infect Dis
; 62(6): 476-80, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19934545
ABSTRACT
Two novel multiplex polymerase chain reaction (mPCR) assays were originally developed for detecting nine targeted virulence-associated genes of five categorized diarrheagenic Escherichia coli (DEC). The mPCR assay 1 included five primer sets (stx1, eaeA, invE, STp gene, and astA), and assay 2 included four primer sets (stx2, aggR, STh gene, and LT gene). The two mPCRs showed 100% specificity in identifying the reference strains without nonspecific bands, and 51 DEC and 38 astA gene-positive E. coli strains from 683 E. coli-like isolates. Our mPCR methods showed high sensitivity and specificity for detecting nine virulence genes of DEC strains. We proved that these methods will contribute to reducing the cost for the reagents of mPCR reported elsewhere and could, therefore, contribute to the diagnosis of DEC in clinical laboratories.
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Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
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Técnicas de Diagnóstico Molecular
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Diarreia
/
Escherichia coli
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Infecções por Escherichia coli
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
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Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Jpn J Infect Dis
Ano de publicação:
2009
Tipo de documento:
Article