Alginate-encapsulated HepG2 cells in a fluidized bed bioreactor maintain function in human liver failure plasma.
Artif Organs
; 33(12): 1117-26, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-20078562
ABSTRACT
Alginate-encapsulated HepG2 cells cultured in microgravity have the potential to serve as the cellular component of a bioartificial liver. This study investigates their performance in normal and liver failure (LF) human plasma over 6-8 h in a fluidized bed bioreactor. After 8 days of microgravity culture, beads containing 1.5 x 10(9) cells were perfused for up to 8 h at 48 mL/min with 300 mL of plasma. After exposure to 90% LF plasma, vital dye staining showed maintained cell viability, while a 7% increase in lactate dehydrogenase activity indicated minimal cell damage. Glucose consumption, lactate production, and a 4.3-fold linear increase in alpha-fetoprotein levels were observed. Detoxificatory function was demonstrated by quantification of bilirubin conjugation, urea synthesis, and Cyp450 1A activity. These data show that in LF plasma, alginate-encapsulated HepG2 cells can maintain viability, and metabolic, synthetic, and detoxificatory activities, indicating that the system can be scaled-up to form the biological component of a bioartificial liver.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Falência Hepática
/
Reatores Biológicos
/
Fígado Artificial
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Alginatos
/
Células Hep G2
Limite:
Humans
Idioma:
En
Revista:
Artif Organs
Ano de publicação:
2009
Tipo de documento:
Article