Porous poly (L-lactic acid) scaffolds are optimal substrates for internal colonization by A6 mesoangioblasts and immunocytochemical analyses.
J Biosci
; 34(6): 873-9, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-20093740
ABSTRACT
In this study, mouse mesoangioblasts were seeded onto bidimensional matrices within three-dimensional porous scaffolds of poly (L-lactic acid) (PLLA), in the presence or absence of a type I collagen coating. The cells were observed under a scanning electron microscope and tested for their adhesion, survival and proliferation. Immunolocalization of heat shock protein (Hsp) 70, an abundant and ubiquitous intracellular protein in these cells, was also performed in sectioned cell-containing scaffolds under a confocal fluorescence microscope to determine if in situ analysis of intracellular constituents was feasible. The data show that PLLA films allow direct cell adhesion and represent an optimal support for cell growth, and that the internal surfaces of PLLA polymeric sponges can be colonized by mesoangioblasts, which can be submitted for in situ confocal microscopic analyses for possible monitoring of timedependent expression of differentiation markers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Imuno-Histoquímica
/
Ácido Láctico
/
Engenharia Tecidual
/
Células Mesangiais
/
Alicerces Teciduais
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
J Biosci
Ano de publicação:
2009
Tipo de documento:
Article