[Molecular characterization of Japanese encephalitis virus isolated from Gansu province in 2008].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(4): 251-3, 2009 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-20108765
ABSTRACT
OBJECTIVE:
To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene.METHODS:
Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4.RESULTS:
The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain.CONCLUSION:
Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encefalite Japonesa
/
Vírus da Encefalite Japonesa (Espécie)
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Ano de publicação:
2009
Tipo de documento:
Article