Comparative cytotoxicity of five root canal sealers on cultured human periodontal ligament fibroblasts.
Int Endod J
; 43(3): 251-7, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-20158537
ABSTRACT
AIM:
To evaluate the cytotoxicity of current root canal sealers to periodontal ligament (PDL) fibroblasts.METHODOLOGY:
Five root canal sealers (Canals, Canals-N, Topseal, Sealapex, Tubliseal) were prepared and placed into transwells. After initial setting for 1 h, the transwells with sealers were placed into cultured PDL fibroblasts. They were cultured for further 3 or 18 h. Morphological changes were observed. Cell viability was estimated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5- diphenyl-tetrazolium bromide (MTT) assay.RESULTS:
Marked retraction and death of PDL fibroblasts were observed after exposure to Canals or Topseal for 3 h. A 3-h exposure of PDL fibroblasts to Tubliseal stimulated MTT reduction. Canals-N showed little cytotoxicity even after an exposure of 18 h.CONCLUSION:
Canals was the most toxic sealer, followed by Topseal. Sealapex and Tubliseal had comparable and moderate cytotoxicity to PDL fibroblasts, whereas Canals-N showed little cytotoxicity. Exposure to Tubliseal may modulate MTT reduction in PDL fibroblasts. Canals-N had good biocompatibility.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
/
Materiais Restauradores do Canal Radicular
Limite:
Humans
Idioma:
En
Revista:
Int Endod J
Ano de publicação:
2010
Tipo de documento:
Article