LINE-1 methylation difference between ameloblastoma and keratocystic odontogenic tumor.
Oral Dis
; 16(3): 286-91, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-20374511
ABSTRACT
OBJECTIVE:
Global hypomethylation is a common epigenetic event in cancer. Keratocystic odontogenic tumor (KCOT) and ameloblastoma are different tumors but posses the same tissue in origin. Here, we investigated long interspersed nuclear element-1 (LINE-1 or L1) methylation status between ameloblastoma and KCOT. MATERIALS ANDMETHODS:
We studied the methylation levels of the long interspersed nucleotide element-1 (LINE-1) in ameloblastoma and KCOT. After collecting ameloblastoma cells and epithelium lining cells of KCOT by laser capture microdissection from paraffin embedded tissue, combined bisulfite restriction analysis of LINE-1 (COBRALINE-1) was performed to measure LINE-1 methylation levels.RESULTS:
The LINE-1 methylation level in KCOT (53.16 +/- 12.03%) was higher than that in ameloblastoma (36.90 +/- 16.52%), with a statistical significance of P = 0.001. The ranges of LINE-1 methylation of both lesions were not associated with either age or sex.CONCLUSION:
We found LINE-1 hypomethylation levels between ameloblastoma and KCOT are different. Therefore, global methylations between these tumors are processed differently.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA de Neoplasias
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Neoplasias Maxilomandibulares
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Tumores Odontogênicos
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Transformação Celular Neoplásica
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Elementos Nucleotídeos Longos e Dispersos
Limite:
Adult
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Aged
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Aged80
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Child
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Oral Dis
Ano de publicação:
2010
Tipo de documento:
Article