mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas.
Clin Biochem
; 43(10-11): 827-35, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20382140
ABSTRACT
BACKGROUND:
Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachidonic acid metabolism, with histological tumor type has not yet been determined. DESIGN ANDMETHODS:
Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure and compare transcript levels of enzymes and receptors implicated in both lipoxygenase and cyclooxygenase pathways between oligodendrogliomas, astrocytomas, glioblastomas and mixed oligoastrocytomas.RESULTS:
Arachidonic acid metabolism-related enzymes and receptor transcripts (i) were underexpressed in classical oligodendrogliomas compared to astrocytomas and/or glioblastomas, (ii) differed between astrocytomas and glioblastomas and (iii) had an intermediate expression in mixed oligoastrocytomas.CONCLUSIONS:
mRNA levels of enzymes and receptors implicated both in lipoxygenase and cyclooxygenase pathways differed significantly in gliomas according to the histological type.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Receptores de Prostaglandina
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Neoplasias do Sistema Nervoso Central
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Ácido Araquidônico
/
Lipoxigenase
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Prostaglandina-Endoperóxido Sintases
/
Glioma
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
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Aged
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Clin Biochem
Ano de publicação:
2010
Tipo de documento:
Article