Accurate and simple sizing of primer extension products using a non-radioactive approach facilitates identification of transcription initiation sites.
J Microbiol Methods
; 81(3): 256-8, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-20385179
ABSTRACT
An improved method of non-radioactive identification of transcription start sites is presented in which the use of 7-deaza dGTP in the primer extension reaction allows the product to be directly aligned to cycle sequencing traces on an automated sequencer. This removes the documented need to apply corrections for mobility differences.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sítio de Iniciação de Transcrição
/
Biologia Molecular
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Microbiol Methods
Ano de publicação:
2010
Tipo de documento:
Article