Evolution and recombination of genes encoding HIV-1 drug resistance and tropism during antiretroviral therapy.
Virology
; 404(1): 5-20, 2010 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-20451945
Characterization of residual plasma virus during antiretroviral therapy (ART) is a high priority to improve understanding of HIV-1 pathogenesis and therapy. To understand the evolution of HIV-1 pol and env genes in viremic patients under selective pressure of ART, we performed longitudinal analyses of plasma-derived pol and env sequences from single HIV-1 genomes. We tested the hypotheses that drug resistance in pol was unrelated to changes in coreceptor usage (tropism), and that recombination played a role in evolution of viral strains. Recombinants were identified by using Bayesian and other computational methods. High-level genotypic resistance was seen in approximately 70% of X4 and R5 strains during ART. There was no significant association between resistance and tropism. Each patient displayed at least one recombinant encompassing env and representing a change in predicted tropism. These data suggest that, in addition to mutation, recombination can play a significant role in shaping HIV-1 evolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Infecções por HIV
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HIV-1
/
Evolução Molecular
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Fármacos Anti-HIV
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Farmacorresistência Viral
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Tropismo Viral
Limite:
Female
/
Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
2010
Tipo de documento:
Article