Effects of intestinal intervention on bacterial translocation in a rat model of acute liver failure in vivo.
Eur J Gastroenterol Hepatol
; 22(11): 1316-22, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-20595903
ABSTRACT
BACKGROUND/AIMS:
The study aimed to confirm bacterial translocation (BT) in rats with acute liver failure (ALF), to explore the correlation between the concentration of lipopolysaccharides and Toll-like receptor 4 (TLR4) expression, and further, to evaluate the curative effects of lactulose and montmorillonite (smecta) in this setting.METHODS:
D-Galactosamine was injected into the abdominal cavity of rats to induce ALF. Escherichia coli JM109 labeled with enhanced green fluorescent protein was administered to track BT. Simultaneously, the rats were given lactulose or smecta. Blood samples were collected for measuring liver function, cytokines, endotoxins, and TLR4 expression. Representative tissue specimens from the liver, spleen, and mesenteric lymph nodes were aseptically harvested for bacterial identification by agarose gel electrophoresis, laser scanning confocal microscopy, and flow cytometry.RESULTS:
BT occurred in ALF, accompanied by impaired liver function with increased cytokines, endotoxins, and TLR4 expression. After the treatment with lactulose or smecta, all these parameters decreased, including the relative quantity of translocated bacteria while albumin increased. Furthermore, compared with the lactulose treatment group, the parameters in the smecta treatment group improved. Moreover, in the group in which smecta was given for prophylaxis, there was greater improvement than with treatment.CONCLUSION:
Intestinal intervention with lactulose or smecta can ameliorate BT; moreover, smecta has a better effect than lactulose, and its preventive effect was also better than its therapeutic effect.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Bentonita
/
Fármacos Gastrointestinais
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Falência Hepática Aguda
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Silicatos
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Translocação Bacteriana
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Escherichia coli
/
Intestinos
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Lactulose
Limite:
Animals
Idioma:
En
Revista:
Eur J Gastroenterol Hepatol
Ano de publicação:
2010
Tipo de documento:
Article