[Roles of proto-oncogene c-erbB2 during the initiation growth of rat primordial follicles].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 26(2): 165-70, 2010 May.
Article
em Zh
| MEDLINE
| ID: mdl-20684269
ABSTRACT
OBJECTIVE:
To study the expression and possible roles of proto-oncogene c-erbB2 during the initiation growth of primordial follicles.METHODS:
Ovaries were collected from 2-day-old SD rats and cultured in the Waymouth culture system. In-situ hybridization, RT-PCR and immunohistochemistry were performed to assess the expressions of c-erbB2 mRNA and protein during the initiation growth of primordial follicles and after the effect of EGF. Western blot was used to observe the PCNA, p-ERK1/2 contents and correlation analysis was used to study the correlation relationship between contents of p-ERK1/2 and expressions of c-erbB2 mRNA at the same time of the primordial follicles growth.RESULTS:
PCNA protein levels appeared to be more intense during the initiation growth of primordial follicles, EGF could promote the proliferation and differentiation of the primordial follicles. c-erbB2 mRNA existed in the oocytes endochylema and ErbB2 existed in the oocytes membrane, the expressions of c-erbB2 mRNA and ErbB2 appeared to be more intense when primordial follicles were cultured for 8 d than cultured for 0 d in the Waymouth culture system and were further increased with 50 ng/ml EGF for 4 d and 8 d. The same results were observed by RT-PCR, too. p-ERK1/2 protein levels were consistent with the changes of c-erbB2 mRNA and protein. Furthermore, Spearman rank correlation analysis showed there was a significant positive correlation relationship between the changes of p-ERK1/2 and the changes of c-erbB2 mRNA during the primordial follicles growth and after the effect of EGF (rs = 0.900, P < 0.05).CONCLUSION:
It was suggested that proto-oncogene c-erbB2 may be play an important role during the initiation growth of primordial follicles with EGF, and it is indirectly suggested that c-erbB2 promotes the development of the primordial follicles via ERK-MAPK signal transduction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ovário
/
Receptor ErbB-2
/
Folículo Ovariano
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ying Yong Sheng Li Xue Za Zhi
Ano de publicação:
2010
Tipo de documento:
Article