Tamoxifen-inducible Cre-mediated recombination in adipocytes.
Genesis
; 48(10): 618-25, 2010 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-20715175
ABSTRACT
To generate a mouse line which allows inducible, Cre/loxP-dependent recombination in adipocytes, we used RedE/RedT-mediated recombineering to insert the CreER(T)²-transgene, which encodes a fusion protein of Cre and a mutated tamoxifen-responsive estrogen receptor, into the start codon of the adipocyte-specific Adipoq gene. Adipoq encodes adiponectin, an adipokine specifically expressed in differentiated adipocytes. Tamoxifen treatment induced almost complete recombination in white adipose tissue of the AdipoqCreER(T)² mouse line (97%-99%), while no recombination was seen in vehicle-treated animals. Recombination in brown adipose tissue was about 15%, whereas other organs and tissues did not undergo recombination. In addition, mice expressing CreER(T)² in adipocytes did not show any alterations of metabolic functions like glucose tolerance, lipolysis, or energy expenditure compared to control mice. Therefore the AdipoqCreER(T)² mouse line will be a valuable tool for studying the consequences of a temporally controlled deletion of floxed genes in white adipose tissue.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Tamoxifeno
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Adipócitos
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Integrases
Limite:
Animals
Idioma:
En
Revista:
Genesis
Ano de publicação:
2010
Tipo de documento:
Article