[Expression of recombinant VP2 gene in insect sf9 cells and screening of clinical specimens].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(6): 427-9, 2009 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-20718346
ABSTRACT
OBJECTIVE:
To clone and express VP, gene from HBoV, and the expressed VP, protein was as the antigen in order to detect serum from children in Wenling area with lower respiratory tract infections.METHODS:
The VP, gene was recombined with the genome of Baculovirus, which infected the insect cell. The fusion protein with HA tag was applied to confirm the specificity of expressed protein. Furthermore, the recombinant protein was observed using electron microscopy. The 176 serum from children in Wenling area with lower respiratory tract infections was screened using Western blot.RESULTS:
The expressed VP2 protein was more than 60% in total proteins from insect cell, and MWt about 60 x 10(3). The virus-like particle (VLP) was observed using electron microscopy, and size about 20 nm. The 176 serum from children in wenling area with lower respiratory tract infections was screened using Western blot. The HBoV positive rate was 2.28% (4/176).CONCLUSION:
The VP2 protein from human bocavirus was expressed in insect cell successfully. Through HA tag the VP2 protein was specific, and then the assay using SDS-PAGE with Western blot could detect and screen the antibody in serum from children with lower respiratory tract infections rapidly and accurately.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Infecções por Parvoviridae
/
Proteínas do Capsídeo
/
Bocavirus
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Ano de publicação:
2009
Tipo de documento:
Article