In vitro and in vivo immunostimulatory activity of Woodfordia fruticosa flowers on non-specific immunity.
Pharm Biol
; 48(9): 1066-72, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20731559
ABSTRACT
CONTEXT Woodfordia fruticosa Kurz. (Lythraceae), a non-rasayana immunomodulatory Indian medicinal plant, used traditionally as an anthelmintic, in dysentery, leprosy, blood diseases, leucorrhea, and menorrhagia. OBJECTIVE:
To investigate the effect of ethanol extract of W. fruticosa flowers on non-specific immune responses in mice. MATERIALS ANDMETHODS:
In vitro immunomodulatory activity of the extract was examined on murine peritoneal macrophage phagocytosis (nitroblue tetrazolium (NBT) dye reduction, lysosomal enzyme activity, nitric oxide and myeloperoxidase) and on proliferation of bone marrow cells by sulforhodamine B (SRB) assay, while the in vivo potential on macrophages and bone marrow cells was evaluated by using carbon clearance test and cyclophosphamide-induced myelosuppression, respectively.RESULTS:
Significant increase in the release of myeloperoxidase, nitric oxide lysosomal enzyme and superoxide from macrophages along with significant increase in phagocytic index in carbon clearance test indicate stimulatory activity of the extract on macrophages. The extract also demonstrated 60% increase in bone marrow cell proliferation and offer protection towards cyclophosphamide-induced myelosuppression which represents the stimulation of bone marrow activity.DISCUSSION:
Significant increase in mediators released from macrophages and phagocytic index in carbon clearance test suggests the release of cytokines from macrophages and stimulation of reticulo-endothelial system. Proliferation of bone marrow cells indicates the plausible release of colony stimulating factors, which further stimulates the immune system through generation of immune cells.CONCLUSION:
The result described here indicates the immunostimulatory activity of ethanol extract of W. fruticosa flowers by stimulating non-specific immune responses, macrophages and bone marrow cells.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Adjuvantes Imunológicos
/
Woodfordia
/
Flores
/
Imunidade Inata
/
Leucopenia
/
Fitoterapia
Limite:
Animals
Idioma:
En
Revista:
Pharm Biol
Ano de publicação:
2010
Tipo de documento:
Article