[Molecular biologic methods for epidemiological precision characterization of disease agents using Salmonella enteritidis from poultry as an example]. / Molekularbiologische Methoden zur epidemiologischen Feincharakterisierung von Krankheitserregern am Beispiel von Salmonella enteritidis aus Geflügel.

ABSTRACT

The principles of molecular biological methods for epidemiological investigation of infectious bacteria are described. They were applied for differentiating S. enteritidis isolates originating from poultry. Among the methods described are the determination of the chromosomal DNA fingerprints (BRENDA), the methods of multilocus enzyme electrophoresis, plasmid profiles, and the outer membrane protein as well as lipopolysaccharide pattern. It turned out, that S. enteritidis strains isolated before 1987, in 1988 and the ones originating from Great Britain did not differ in anyone of these criteria. Consequently one has to assume, that the strains recently causing problems originate from the population of S. enteritidis which had existed in poultry before.