Identification of hypoxanthine and guanine as the co-repressors for the purine regulon genes of Escherichia coli.
Mol Microbiol
; 4(12): 2187-92, 1990 Dec.
Article
em En
| MEDLINE
| ID: mdl-2089227
ABSTRACT
Addition of purine compounds to the growth medium of Escherichia coli and Salmonella typhimurium causes repressed synthesis of the purine biosynthetic enzymes. The repression is mediated through a regulatory protein, PurR. To identify the co-repressor(s) of PurR, two approaches were used (i) mutations were introduced into purine salvage genes and the effects of different purines on pur gene expression were determined; (ii) purine compounds which dictate the binding of the PurR protein to its operator DNA were resolved by gel retardation. Both the in vivo and the in vitro data indicated that guanine and hypoxanthine are co-repressors. The toxic purine analogues 6-mercaptopurine and 6-thioguanine also activated the binding of PurR to its operator DNA.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Proteínas de Bactérias
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Guanina
/
Hipoxantinas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Mol Microbiol
Ano de publicação:
1990
Tipo de documento:
Article