Isolation of murine adipose-derived stem cells.
Methods Mol Biol
; 702: 29-36, 2011.
Article
em En
| MEDLINE
| ID: mdl-21082392
ABSTRACT
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Primary adipocytes grown in culture and derived from murine adipose tissue are essential for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stem cells along with a protocol for inducing adipogenesis in this cell population.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Separação Celular
/
Tecido Adiposo
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2011
Tipo de documento:
Article