Fluoride induces apoptosis and alters collagen I expression in rat osteoblasts.
Toxicol Lett
; 200(3): 133-8, 2011 Feb 05.
Article
em En
| MEDLINE
| ID: mdl-21093551
In this study we investigated apoptosis and expression of the collagen I gene in newborn rat osteoblasts (OB) by the administration of varying concentrations of fluoride (F). Sodium fluoride (NaF) at concentrations of 0, 0.5, 5, 10, and 20mg/L was administered to cultured OB. The percentage of G(1)/G(0) (Gap 1/Gap 0), S (synthesis), G(2)/M (Gap 2/M, mitosis), and apoptosis rates in OB were analyzed with a Fluorescence-activated Cell Sorter (FACS) by propidium iodine (PI) staining after F treatment of 72 h. Effects of F treatment on COL1A1 and COL1A2 mRNA and collagen I protein levels were determined using quantitative real time RT-PCR (qRT-PCR) and immunofluorescence, respectively. This study demonstrates that there is a pronounced negative effect of long term NaF treatment on OB survival. These negative effects include an inhibition of the transformation from S phase into G(2)/M phase, increased apoptosis, and decreased COL1A1 mRNA, down-regulating the synthesis of COL I protein. The results suggest that COL I protein degradation in OB from F toxicity is due to a depletion of COL1A1 mRNA and not COL1A2.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
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Fluoreto de Sódio
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Apoptose
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Colágeno Tipo I
Limite:
Animals
Idioma:
En
Revista:
Toxicol Lett
Ano de publicação:
2011
Tipo de documento:
Article