Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene.
Mol Syst Biol
; 6: 440, 2010 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-21119634
ABSTRACT
The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question in the absence of external perturbation, how long will a cell stay in the lysogenic state before spontaneously switching away from that state? We show by direct measurement that lysogen stability exhibits a simple exponential dependence on the frequency of activity bursts from the fate-determining gene, cI. We quantify these gene-activity bursts using single-molecule-resolution mRNA measurements in individual cells, analyzed using a stochastic mathematical model of the gene-network kinetics. The quantitative relation between stability and gene activity is independent of the fine details of gene regulation, suggesting that a quantitative prediction of cell-state stability may also be possible in more complex systems.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Regulação Bacteriana da Expressão Gênica
/
Instabilidade Genômica
/
Proteínas Virais Reguladoras e Acessórias
/
Lisogenia
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Mol Syst Biol
Ano de publicação:
2010
Tipo de documento:
Article