[The changes of cAMP and adenylyl cyclase mRNA level in hippocampus of mice with ischemia/reperfusion].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 21(3): 278-80, 2005 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-21162195
ABSTRACT
AIM:
To observe the cAMP and adenylyl cyclase (AC) mRNA level in hippocampus of mice with vascular dementia (ischemia/reperfusion), and explore the molecular pathogenesis of ischemia/reperfusion.METHODS:
The mice were subjected for ischemia/reperfusion three times on bilateral common carotid arteries by knots to establish models of ischemia/reperfusion and the changes of learning and memory were tested on 29 d/30 d after operation. Sham-operation mice were introduced as control group. The cAMP level was evaluated by the radioimmunoassay (RIA), AC mRNA positive neurons of hippocampus CA1 area were dyed through in-situ hybridization (ISH).RESULTS:
Compared with sham-operation group, the learning and memory of model group was worse (P < 0.05), the cAMP level in hippocampus was lower (P < 0.05) and the surface density (Sv) of AC mRNA positive neurons reduced (P < 0.05).CONCLUSION:
The lower cAMP and AC mRNA level in hippocampus might participate in the molecular pathogenesis of ischemia/reperfusion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Adenilil Ciclases
/
AMP Cíclico
/
Hipocampo
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ying Yong Sheng Li Xue Za Zhi
Ano de publicação:
2005
Tipo de documento:
Article