Activation of TRPV4 channels reduces migration of immortalized neuroendocrine cells.
J Neurochem
; 116(4): 606-15, 2011 Feb.
Article
em En
| MEDLINE
| ID: mdl-21166676
ABSTRACT
Calcium is a universal signal, and its capacity to encode intracellular messages via spatial, temporal and amplitude characteristics allows it to participate in most cellular events. In a specific context, calcium plays a pivotal role in migration, although its role has not been elucidated fully. By using immortalized gonadotropin-releasing hormone-secreting neurons (GN11), we have now investigated the role of TRPV4, a member of the vanilloid family of Ca(2+) channels, in neuronal migration. Our results show that TRPV4 channels are present and functional in GN11 cells and their localization is polarized and enriched in lamellipodial structures. TRPV4 activation leads to a retraction of the lamellipodia and to a decrease in migratory behaviour; moreover cells migrate slower and in a more random manner. We therefore provide evidence for a new regulation of gonadotropin-releasing hormone neurons and a new role for calcium at the leading edge of migratory cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibição de Migração Celular
/
Canais de Cátion TRPV
/
Células Neuroendócrinas
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
2011
Tipo de documento:
Article