Occurrence of a silicatein gene in glass sponges (Hexactinellida: Porifera).
Mar Biotechnol (NY)
; 13(4): 810-9, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21181423
ABSTRACT
Silicatein genes are involved in spicule formation in demosponges (Demospongiae Porifera). However, numerous attempts to isolate silicatein genes from glass sponges (Hexactinellida Porifera) resulted in a limited success. In the present investigation, we performed analysis of potential silicatein/cathepsin transcripts in three different species of glass sponges (Pheronema raphanus, Aulosaccus schulzei, and Bathydorus levis). In total, 472 clones of such transcripts have been analyzed. Most of them represent cathepsin transcripts and only three clones have been found to represent transcripts, which can be related to silicateins. Silicatein transcripts were identified in A. schulzei (Hexactinellida; Lyssacinosida; Rosselidae), and the corresponding gene was called AuSil-Hexa. Expression of AuSil-Hexa in A. schulzei was confirmed by real-time PCR. Comparative sequence analysis indicates high sequence identity of the A. schulzei silicatein with demosponge silicateins described previously. A phylogenetic analysis indicates that the AuSil-Hexa protein belongs to silicateins. However, the AuSil-Hexa protein contains a catalytic cysteine instead of the conventional serine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Poríferos
/
Conformação Proteica
/
Catepsinas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Mar Biotechnol (NY)
Ano de publicação:
2011
Tipo de documento:
Article